data from "Environmental Drivers of the Moonmilk Microbiome Diversity in Some Temperate and Tropical Caves" by Theodorescu et al. (https://link.springer.com/article/10.1007/s00248-023-02286-8)
20 samples
~10-20mb
PRJEB63212 (European Nucelotide Archive)
"Moonmilk samples were collected from four caves, three in Romania: Ferice and Fața Apei caves located in Apuseni Mountains (north-western Romania), Izvorul Tăușoarelor Cave (Rodnei Mountains, northern Romania), and one in south-eastern Brazil: Nestor Cave from north Minas Gerais State."
The accession numbers are found in the data directory in the script called sequences.sh
This study used the V3-V4 region of the 16S gene with 314F and 805R primers (they cited a paper with a method that I dont have access to so I am imaging this is the sequence): 16S Amplicon PCR Forward Primer 314F = 5' CCTACGGGNGGCWGCAG 16S Amplicon PCR Reverse Primer 805R= 5' GACTACHVGGGTATCTAATCC